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KMID : 0438419970040010042
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Korean Journal of Bone Metabolism
1997 Volume.4 No. 1 p.42 ~ p.49
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The Effect of Sodium Fluoride on the Mineralized Nodule Formation and Osteblatic Cell
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Chae Han-Jung
Kang Jang-Sook
Kim Dae-Eop
Kim Hyung-Ryong
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Abstract
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The clinical use of fluoride with a well known osteogenic action in osteoporotic patients is rational, because this condition is characterized by impaired bone formation. However, its anabolic effect has not been demonstrated well in vitro. To study the effects of fluoride on overall bone metabolism, we isolated culture system of osteoblastic cells from fetal rat calvaria. Considerable number of mineralized nodules were shown in osteoblastic cell cultures, which had been maintained in the presence of ascorbic acid and beta-glycerophosphate up to 21 days. The number of mineralized nodules was not increased significantly by continuous treatment of cultures with 10muM NaF, a clinically effective osteogenic concentration. When cultures were treated with pulses of 48 hr duration before apparent mineralization was occurring, 2-fold increase in their number was detected. Alkaline phosphatase activity of osteoblastic cells was inhibited by Sodium Fluoride in dose-dependent manner. Sodium fluoride stimulated proliferation in osteoblastic cells. The effect of Sodium Fluoride on the cell proliferation was measured by the incorporation of [3H]-thymidine into DNA. Aa a result, NaF at 1∼100muM increased the [3H]-thymidine incorporation into DNA in a dose dependent manner. These results taken together suggest that fluoride enhances bone formation and that the stimulatory effect to fluoride on the proliferation of osteoblatic cells is probably most relevant to its mechanism underlying augmented bone formation.
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